Flow Cytometer with Cell Sorter
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. Interests are first labeled with an antibody which is individual for a particular cell surface molecule. Antibody is coupled to a fluorescent dye, like when in a narrow stream the individual cells pass a laser beam in single file, the fluorescence of each cell is measured. A vibrating nozzle then forms small droplets which each containing a single cell which are given a negative or positive charge based on whether the cell they contain is fluorescing. A strong electric field defects the various charged droplets into separate containers so that each container has a homogeneous population of cells eventually with respect to the cell surface molecule tagged along fluorescent antibody.
- Cell cloning.
- Chromosome sorting.
- Genetic disease diagnosis.
- Cancer stem cell analysis, etc.,
|Detection||FSC, SSC, Up to 9 fluorescence detection channels with optional changeable filters|
|Absorbance/Excitation||488 nm, 640 nm, 405 nm, 561 nm|
|Excitation Wavelength||405 nm (Violet), 488 nm (Blue), 640-nm (Red)|
|Sensitivity||30 molecules of equivalent soluble fluorochrome PE (MESF-PE), 80 MESF-FITC (lower numbers indicate higher system sensitivity)|
|Resolution||Full peak coefficient of variation (FPCV) of < 3.0%|
|Yield & Purity||> 80% &> 98%|
|Throughput||10,000 cells/second; 40,000 events per
second; 2-way sorting
|Sample Type||1.5-, 2.0- and 5.0-mL tubes (two-way sorting); Up to 96-wells (one-way sorting)|
|Sample Volume Input||5 mL tubes; Two-way sorting: 1.5, 2.0 & 5.0-mL tubes
One-way sorting: 6, 24, 48, 96 & 384-well plates, 96-well PCR tray
|Flow Rate||34,000 drops/second|
|Software||BD FACS Chorus version 1.0 or later|
|Laser Type||Class 1 Laser Product per IEC/EN 60825-1.|
Details of Flow Cytometer with Cell Sorter
|Brand||BD- Becton, Dickinson and Company, Singapore Model|
|Model||:BD FACSMelody™ Sorter (661762)|
|Sponsored Agency||DST- PURSE programme (Phase -2) (Sanction Order No- SR/PURSE Phase 2/16 (C)/2017)|
|Name of the Instrument||BDU Departments||Affiliated Colleges||Other Universities / Institutes||Industries / Non-academics|
|Flow Cytometer with Cell Sorter||1000||1500||2000||2500|
* Additional 18% GST.