University Science Instrumentation Centre (USIC)
Bharathidasan University
Tiruchirappalli - 620 024, Tamil Nadu, India

Flow Cytometer with Cell Sorter

Working Principle

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. Interests are first labeled with an antibody which is individual for a particular cell surface molecule. Antibody is coupled to a fluorescent dye, like when in a narrow stream the individual cells pass a laser beam in single file, the fluorescence of each cell is measured. A vibrating nozzle then forms small droplets which each containing a single cell which are given a negative or positive charge based on whether the cell they contain is fluorescing. A strong electric field defects the various charged droplets into separate containers so that each container has a homogeneous population of cells eventually with respect to the cell surface molecule tagged along fluorescent antibody.

flow cytometer

Key Applications

  •   Cell cloning.
  •   Chromosome sorting.
  •   Genetic disease diagnosis.
  •   Cancer stem cell analysis, etc.,

Technical Specifications

Detection FSC, SSC, Up to 9 fluorescence detection channels with optional changeable filters
Absorbance/Excitation 488 nm, 640 nm, 405 nm, 561 nm
Excitation Wavelength 405 nm (Violet), 488 nm (Blue), 640-nm (Red)
Sensitivity 30 molecules of equivalent soluble fluorochrome PE (MESF-PE), 80 MESF-FITC (lower numbers indicate higher system sensitivity)
Resolution Full peak coefficient of variation (FPCV) of < 3.0%
Yield & Purity > 80% &> 98%
Throughput 10,000 cells/second; 40,000 events per
second; 2-way sorting
Sample Type 1.5-, 2.0- and 5.0-mL tubes (two-way sorting); Up to 96-wells (one-way sorting)
Sample Volume Input 5 mL tubes; Two-way sorting: 1.5, 2.0 & 5.0-mL tubes
One-way sorting: 6, 24, 48, 96 & 384-well plates, 96-well PCR tray
Flow Rate 34,000 drops/second
Software BD FACS Chorus version 1.0 or later
Laser Type

Class 1 Laser Product per IEC/EN 60825-1.

Details of Flow Cytometer with Cell Sorter

BrandBD- Becton, Dickinson and Company, Singapore Model
Model:BD FACSMelodyâ„¢ Sorter (661762)
Sponsored AgencyDST- PURSE programme (Phase -2) (Sanction Order No- SR/PURSE Phase 2/16 (C)/2017)

Tariff Details

Name of the Instrument BDU Departments Affiliated Colleges Other Universities / Institutes Industries / Non-academics
Flow Cytometer with Cell Sorter  1000  1500  2000  2500

* Additional 18% GST.