University Science Instrumentation Centre (USIC)
Bharathidasan University
Tiruchirappalli - 620 024, Tamil Nadu, India

Spectrofluorometer with TCSPC

Working Principle

Spectrofluorometers (or fluorescence spectrophotometers) measure the fluorescence signature of an analyte in a sample based on its specific excitation and emission wavelengths. The fluorescence signature can be correlated to the concentration level of the analyte in the sample. The most compelling reason for using a double monochromator on a spectrofluorometer is to reduce the stray light level. Stray light usually refers to any radiation at wavelengths other than the selected wavelength, which may fluorescence. A photomultiplier tube or PMT is a type of vacuum phototube. It is a highly sensitive detector of light in the ultraviolet, visible, and near-infrared ranges of the electromagnetic spectrum.


Specific Features of TCSPC

  •   A fluorometer measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength. All fluorescent objects have its own fingerprint, meaning that it excites and emits predictable types of light.
  •   Time‐correlated single‐photon counting (TCSPC) is considered the most sensitive method for determining fluorescence lifetimes. It is a digital technique, with well defined (Poisson) statistics, which is not affected by changes in source intensity, unlike some analogue measurement techniques. TCSPC is based on the detection of the arrival times of individual photons after optical excitation of a sample. It makes use of a pulsed excitation source (typically laser or LED)


  •   Water Raman signal-to-noise ratio: 10,000:1 (FSD method)
  •   Photon Counting for Ultimate Sensitivity
  •   Fast Scanning capability - up to 80 nm/second
  •   Phosphorimeter accessory for long-lived luminescence determinations
  •   Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  •   Measure lifetimes from 25ps with laser diodes and PPD detection module
  •   Up to 1,000 TCSPC measurements per second – ideal for kinetics studies
  •   Monochromator wavelength selection
  •   Measurement modes
  •   Lifetime – measure 25ps to 1s
  •   Kinetic TCSPC – 1 to 10 000 decays measured sequentially in 1ms to 1min per decay
  •   Anisotropy – reconvolution analysis to resolve shorter rotational correlation times
  •   Time-Resolved Emission Spectra (TRES) – Collect up to 100 wavelength dependent decays
  •   Steady-State


  •   Fluorescence spectroscopy includes the study of fluorescent dyes that are widely used with biological samples, both in routine assays and in advanced research. It is also employed in material science to characterize luminescent materials.
  •   The standard in both steady state and time-resolved photoluminescence spectroscopy for both fundamental research and routine laboratory applications.
  •   Time Correlated Single-Photon Counting (TCSPC) is the method of choice for fluorescence lifetime measurements. The TCSPC technique outperforms all other techniques in sensitivity, dynamic range, data accuracy and precision.

Details of Spectrofluorometer with TCSPC

BrandHoriba, Japan
ModelFluoroMax Plus
Sponsored AgencyDST- PURSE programme (Phase -2) (Sanction Order No- SR/PURSE Phase 2/16 (C)/2017)

Tariff Details

Name of the Instrument BDU Departments Affiliated Colleges Other Universities / Institutes Industries / Non-academics
SpectroFluorometer with TCSPC  250  300  500  1000

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